In vitro Antioxidant Activities and Effect of Hydroethanolic and Aqueous Extracts of Terminalia avicennioides (Combretaceae) on Salmonella

Main Article Content

Louis-Claire Ndel Famen
Benjamin Tangue Talom
Richard Simo Tagne
Gabriel Tchuente Kamsu
Norbert Kodjio
Stephen Tamekou Lacmata
Donatien Gatsing

Abstract

Today, Typhoid fever remains a public health problem in developing countries due to the poor quality of lifestyle associated with abusive and inappropriate use of antibiotics.

Aims: Considering the ethnopharmacological relevance of Terminalia avicennioides                              (T. avicennioides) (Combretaceae), this study was designed to investigate the in vitro antisalmonella and antioxidant activities of various extracts of this plant.

Methodology: The microdilution method was used to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of T. avicennioides extract. These extracts were also subjected to in vitro antioxidant tests such as diphényl-2-picrylhydrazyle (DPPH) radical scavenging test, ferric reducing-antioxidant power (FRAP), hydroxyl radical (OH) nitric oxide (NO) and Hydrogen Peroxide Scavenging Capacity.

Results: In vitro antisalmonella activity reveals that T. avicennioides stem bark extracts presented MIC values ranging from 64 to 512 μg / mL on tested microorganisms. This extract exhibited a good ability to trap DPPH with an IC50 of 8.30 μg / mL. The iron reducing power obtained with this extract had ODs ranging from 0.96 to 1.63. Phytochemical screening showed the presence of alkaloids, flavonoids, saponins, phenols anthocyanin and anthraquinone in all the extracts.

Conclusion: The results suggest that stem extract of T. avicennioides contains antisalmonella and antioxidant substances, which could be used for the treatment of typhoid fever and another salmonellosis.

Keywords:
Terminalia avicennioides, antisalmonella activity, antioxidant activity, phytochemical screening.

Article Details

How to Cite
Ndel Famen, L.-C., Tangue Talom, B., Simo Tagne, R., Tchuente Kamsu, G., Kodjio, N., Tamekou Lacmata, S., & Gatsing, D. (2020). In vitro Antioxidant Activities and Effect of Hydroethanolic and Aqueous Extracts of Terminalia avicennioides (Combretaceae) on Salmonella. Microbiology Research Journal International, 30(1), 1-14. https://doi.org/10.9734/mrji/2020/v30i130185
Section
Original Research Article

References

ECDC (Centers for Disease Control and Prevention). Typhoid and Paratyphoid Fever; 2015.
Available:https://www.ecdc.europa.eu/en/typhoid-and-paratyphoid-fever, 12 juin,

World Health Organization Typhoid: Key facts; 2018.
Available:https://WWW.Who.int/mediacentre/factsheets/typhoid/en/ 6 mars, 2019

WHO (World Health Organization). Relevé épidémiologique mensuel, N° 16/02, bureau de la représentation du Cameroun, Yaoundé-Cameroun, Juillet. 2016;10.

Vikesland P, Garner E, Gupta S, Kang S, Maile-Moskowits, Zhu N. Differential drivers of antimicrobial resistance across the world. Accounts of Chemical Research. 2019;52:916-924.

Yang L, Mih N, Anand A, Park JH, Tan J, Yurkovich JT, MonK JM et al. Cellular responses to reactive oxygen species are predicted from molecular mechanisms. Proceedings of the National Academy of Sciences. 2019;116(28):14368-14373.

Mann A, Yahaya Y, Banso A, John, F. Photochemical and antimicrobial activity of Terminalia avicenniodes associated with patients suffering from complicated respiratory tract disease. Journal of Medicinal Plant Research. 2008;2(5):094-097.

Duke JA. Le Pouvoir des Plantes. 2e Ed. Encyclopédie des Plantes Médicinales du Département de Phytothérapie de Bobigny, France; 2000.
[ISBN : 2-03- 560252-1] French

Mativandlela SPN, Lall N, Meyer JJM. Antibacterial, antifungal and antitubercular activity of Pelargonium reniforme (CURT) and Pelargonium sidoides (DC) (Geraniaceae) root extracts. South African Journal of Botany. 2006;72:232–237.

Mensor LL, Menezes FS, Leitao GG, Reis ASO, Dos Santos TC, Coube, CS, Leitao, SG Screening of Brazilian plant extracts for antioxidant activity by the used DPPH free radical method. Phytotherapy Research. 2001;15:127-130.

Mesfin T, Aberra G, Asfaw D. In vitro anti-Neisseria gonorrhoeae activity of Albizia gummifera and Croton macrostachyus in Revista CENIC. Ciencias Biológicas, Centro Nacional de Investigaciones Científicas, Cuba. 2010;41:1-11.

Yu W, Zhao Y, Shu B. The radical scavenging activities of radix puerriae iso flavonoids: A chemiluminescence study. Food Chemistry. 2004;86:525-529.

Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite and 15N nitrate in biological fluids. Analysis Biochemistry. 1982;126:131-8.

Chanda S, Dave R. In vitro models for antioxidant activity evaluation and some medicinal plants possessing antioxidant properties: An overview. Africa Journal Microbiology Research. 2009;3: 981-96.

Ruch RJ, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intracellular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis. 1989;10:1003-1008.

Ramde-Tiendrebeogo A, Tibiri A, Hilou A, Lompo M, Millogo-Kone H, Nacoulma OG, Guissou IP. Antioxidative and antibacterial activities of phenolic compounds from Ficus sue Forssk. International Journal of Biological and Chemical Sciences. 2012; 6(1):328-336.

Padmaja M, Sravanthi M, Hemalatha KPJ. Evaluation of antioxidant activity of two Indian medicinal plants. Journal of Phytology. 2011;3(3):86-91.

Harbone JB. Phytochemical methods: A guide to modern techniques of plants analysis. London: Chapman and Hall Ltd. 1973;50-116.

Bolou GEK, Attioua B, N’Guessan AC, Coulibaly A, N’Guessan JD, Djaman AJ. Évaluation in vitro de l’activité antibactérienne des extraits de Terminalia glaucescens Planch. Sur Salmonella Typhi et Salmonella Typhimurium. Bulletin de la Société Royale des Sciences de Liège. 2011;80:772 -790.

Kuete V. Potential of cameroonian plants and derived products against microbial infections: A Review. Planta Medica. 2010; 76:1-13.

Cowan MC. Plant Products as Antimicrobial Agents. Clinical Microbiology Reviews. 1999;12:564-582.

Brunetton J. Pharmacognosie, phytochimie, plantes médicinales (3Edn). Lavoisier Tec et Doc: Paris. 1999;1120.

Foutse Y, Mouokeu RS, Nguimatsia F, Njateng GSS, Tamokou JD, Kuiate JR. Antibacterial activity of methanol extracts and fractions from Kalanchoe crenata, Terminalia avicennioides and Sarcocephalus latifolius. Phamacologia. 2014;5(6):199-204.

Musa FM, Ameh JB, Ado SA, Olonitola OS. Evaluation of phytochemical and antibacterial properties of Terminalia avicennioides crude extract against selected bacteria from diarrhoeic patients. Bajopas. 2016;9(1):131-132.

Gulcin I, Huyut Z, Elmastas M, Aboul-Enein HY. Radical scavenging and antioxidant activity of tannic acid. Arabian Journal of Chemistry. 2010;3:43-53.

De Pooter HL, Schamp N. Comparaison of the volatils composition of some Calamintha satureja species. In: Progress in Essential Oil Research. Ed. E-J. Brunk, Walter De Gruyter. Berli. 1986;139-150.

Ćetković GS, Čanadanović-Brunet J, Djilas SM, Tumbas VT, Markov SL, Cetković DD. Atioxidant potential, lipid peroxidation inhibition and antimicrobial activities of Satureja Montana L. subsp. Kitaibelii Extracts. International Journal of Molecular Sciences. 2007;8(10):1013-1027.

Souri E, Amin G, Farsam H, Barazandeh TM. Screening of antioxidant activity and phenolic content of 24 medicinal plants. Journal of Pharmaceutical Sciences. 2008; 16(2):83-87.

Palash M, Tarum K, Mitali G. Free radical scanvening activity and phytochemical analysis in the leaf and stem of Drymania diandra Blume. International Journal of Integrative Biology. 2009;2:82-83.

Sousa A, Ferreira ICFR, Barros L, Bento A, Pereira JA. Antioxidant potential of traditional stoned table olives ‘‘Alcaparras”: influence of the solvent and temperature extraction conditions. LWT Food Science Technology. 2008;41:739-745.

Duh PD, Du PC, Yen GC. Action of methanolic extract of mung beans hulls as inhibitors of lipid peroxidation and non-lipidoxidative damage. Food Chemistry Toxicology. 1999;37:1055-1061.

Duh PD. Antioxidant activity of burdock (Arctiumlappa linne.): Its scavenging effect on free-radical and active oxygen. Journal of the American Oil Chemists' Society. 1998;75:455-461.

Mathew S, Abraham TE. In vitro antioxidant activities and scavenging effects of Cinnamomum verum leaf extract assayed by different methodologies. Journal Food Chemical Toxicology. 2006; 44:198-206.

Contreras-Guzman ES et Strong FC. Determination of tocopherols (vitamin E) by reduction of cupric ion. Journal Association of official Analytical Chemists. 1982;65:1215-1221.

Vinson JA, Yong H, Xuchui S, Zubik L. Phenol antioxidant quantity and quality in foods: vegetables. Journal Agriculture Food Chemistry. 1998;46: 3630-3634.

Yadav S, Gupta VK, Gopalakrishnan A, Verma R. Antioxidant activity analysis of Ficus racemosa leaf extract. Journal of Entomology and Zoology Studies. 2019; 7(1):1443-1446.

Yen GC, Duh PD, Tsai CL. The relationship between antioxidant activity and maturity of peanut hulls. Journal Agriculture Food Chemistry. 1993;41:67 70.

Umamaheswari, Chatterjee TK. In vitro antioxidant activities of the fractions of Cocciniagrandis L. leaf extract. Africa Journal Traditional Complement Alternative Medicine. 2008;5(1):61-73.