Using of Some Agro-industrial Wastes for Improving Carotenoids Production from Yeast Rhodotorula glutinis 32 and Bacteria Erwinia uredovora DSMZ 30080

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Gehan F. Galal
Rania F. Ahmed

Abstract

Some agro-industrial wastes such as clarified cane molasses, high test molasses, sweet whey, potato starch and corn steep liquor were tested as carbon sources or nitrogen source for growth and carotenoid accumulation using bacteria Erwinia uredovora DSMZ 30080 and yeast Rhodotorula glutinis number 32. Erlenmeyer flasks containing 100 ml of production media, the flasks were inoculated with 1 ml of standard inoculum and incubated at 150 rpm for 4 days at 30°C. Samples were collected periodical every 24h, cell dry weight and carotenoids concentration were determined. Sweet whey and highest molasses gave the highest growth being 2.85 and 7.34 gl-1, respectively and scored the same layout on carotenoids conc. which reach the peak during stationary phase (72 h of fermentation). Using of high test molasses and sweet whey as carotenoid production media were incremented carotenoid conc. about 1.7 and 2 fold (with respect to reference media). Increasing high test molasses conc. to give 5% initial sugar led to up great growth, carotenoids conc., productivity, yield and Yc/x from Rhodo. glutinis 32 to be 7.31 gl-1, 2.67 mgl-1, 0.037 mgl-1h-1, 0.067% and 0.365, respectively. Furthermore, using corn steep liquor (30%) as nitrogen sources augmented carotenoids concentration about 3.8 and 4fold for incomplete and complete production media using Rhodo. glutinis32. Also, a negligible effect on growth was observed with dark incubation with both strains which dropped about 75 and 48% with regard to control for E. uredovora DSMZ 30080 and Rhod. glutinis 32, respectively, whereas, carotenoids conc. was increased about 21% for E. uredovora DSMZ 30080 in dark condition.

Keywords:
Carotenoids production, Rhodotorula glutinis 32, Erwinia uredovora DSMZ 30080, high test molasses, sweet whey.

Article Details

How to Cite
Galal, G. F., & Ahmed, R. F. (2020). Using of Some Agro-industrial Wastes for Improving Carotenoids Production from Yeast Rhodotorula glutinis 32 and Bacteria Erwinia uredovora DSMZ 30080. Microbiology Research Journal International, 30(1), 15-25. https://doi.org/10.9734/mrji/2020/v30i130186
Section
Original Research Article

References

Zollinger H. Color chemistry: syntheses, properties, and applica-tions of organic dyes and pigments: 3rd Ed., Helvetica: Chimica Acta, Zurich; 2003.

Babitha S, Soccol CR, Pandey A. Jackfruit Seed- A novel substrate for the production of Monascus pigments through solid-state fermentation. Food Technol. Biotchnol. 2006;44:465–471.

Park P, Cho D, Kim E, Chu KH. Optimization of carotenoid production by Rhodotorula glutinis using statisti-cal experimental design. World J. Microbiol. Biotechnol. 2005;21:429-434.

Cabral MMS, Cence K, Zeni J, Tsai M, Durrer A, Foltran LL, et al. Caroten-oids production from a newly isolated Sporidiobolus pararoseus strain by submerged fermentation. Eur. Food. Res. Technol. 2011;233:159-166.

Frengova GI Beshkova DM. Caroten-oids from Rhodotorula and Phaffia: yeasts of biotechnological importance. J. Ind. Microbiol. Bio-technol. 2009;36: 163–180.

Gomez LCM, Montanez J, Zavala AM, Aguilar CN. Biotechnological produc-tion of carotenoids by yeasts: An overview. Microb. Cell Fac. 2014;13: 12-20.

Selim AEI, Kassem M M, Bayoumy SMM, El- Sawah MMA Ali DFI. Effect of some sugars and agro-industrial byproduct on carotenoids production by some yeast strains of Rhodotorula spp. J. Plant Production. 2013;4: 1079-1107.

Malisorn C, Suntornsuk W. Optimization of β-carotene production by Rhodotorula glutinis DM28 in fermented radish brine. Bioreso. Technol. 2008;99:2281–2287.

Dannert CS. Engineering novel carotenoids in microorganisms. Curr. Opin. Biotechnol. 2000;11:255-261.

Dannert CS, Umeno D, Arnold FH. Molecular breeding of carotenoid bio-synthetic pathways. Natur. Biotechnol. 2000;18:750–753.

Shabtai Y, Mukmenev I. Enhanced production of pigment-free pullulan by a morphogenetically arrested Aureobasidium pullulans (ATCC 42023) in a two-stage fermentation with shift from soy bean oil to sucrose. Appl. Microbiol. Biotechnol. 1995; 43:595–603.

Frengova GI, Simova ED, Pavlov K, Beshkova DM, Grigorova D. Forma-tion of carotenoids by Rhodotorula glutinis in whey ultra-filtrate. Bio-technol. Bioeng. 1994;44:888–894.

Painter PR, Marr AG. Mathematics of microbial populations. Annual Rev. Microbiol. 1963;22:219-221.

Stanier RY, Doudoroff M, Adelberg EA. General microbiology, 3rd Ed., Macmillan & Co. Ltd.: London; 1970.

Ramadan EM, El-Sawy M, Gamal RF, Abd El-Hady HM. Growth parameters of yeast grown on agricultural residues using shake flask as a batch culture. Annl. Agric. Sci. 1985;30:161-173.

Grothe E, Moo-Young M, Chisti Y. Fermentation optimization for the production of poly (β-hydroxybutyric acid) microbial thermoplastic. Enzyme and Microb. Technol. 1999;25:132-141.

Lee SY. Plastic bacteria? Progress and prospects for polyhydroxyal-kanoate production in bacteria. Trends Biotechnol. 1996;14:431- 438.

El-Banna AA, Abd El-Razek AM, El-Mahdy AR. Some factors affecting the production of carotenoids by Rhodotorula glutinis var. glutinis. Food Nutrit. Sci. 2012;3:64-71.

Goodwin TW. The biochemistry of the carotenoids. Plants, 1st Ed., Chapman Hall: London; 1980.

Ferrao M, Garg S. Studies on effect of media components on growth and β-carotene production by Rhodotorula graminis RC04, J. Cell Tissue Res. 2011;11:2551-2556.

Panesar R, Kaur S, Panesar PS. Production of microbial pigments utilizing agro-industrial waste: A review. Curr. Opinion Food Sci. 2015; 1:70-76

Stachowiak B, Czarnecki Z. Effect of light on carotenoid yield in fed culture of Phffia rhodozyma CBS 5626. Pol. J. Food Nutr. Sci. 2007;57: 129-131.

Khodaiyan F, Hadi razavi S, Emam-djomeh Z, Mousavi SMA, Hejazi MA. Effect of culture conditions on canthaxanthin production by Dietzia natronolimnaea HS-1.J. Microbiol. Biotechnol. 2007;17:195–201.