Occurrence of Enterobacteriaceae with Serological Cross Reactivity towards Salmonella spp., Shigella spp. and Vibrio cholerae in Food

Main Article Content

Md. Fakruddin
Md. Mizanur Rahaman
Monzur Morshed Ahmed
Md. Mahfuzul Hoque

Abstract

Aims: This study was conducted to determine the occurrence of serologically cross-reactive enterobacteriaceae in food samples.
Place and Duration of Study: This study was conducted in Industrial Microbiology Laboratory, IFST, BCSIR, Dhaka, Bangladesh during January-November, 2013.
Methodology: 14 Enterobacteriaceae was isolated from food samples and identified. Serological reaction was determined by slide agglutination method.
Results: Among 14 isolates, 6 were identified to be Cronobacter spp., 3 to be Shigella spp., 2 to be Enterobacter spp., 2 to be Escherichia coli and 1 as Klebsiella spp. Three of the isolates belonging to Cronobacter showed extremely strong cross-reactivity with Salmonella spp., Shigella spp. and Vibrio cholerae. Other isolates gave relatively weak but significant cross-reactions. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) of cell surface protein also showed similar bands in the isolates as type strains of Salmonella, Shigella and Vibrio cholerae.
Conclusion: The results indicate that Salmonella spp., Shigella spp. and Vibrio cholerae surface antigens are shared by Enterobacteriaceae present in food.

Keywords:
Enterobacteriaceae, serological, cross-reactivity, food

Article Details

How to Cite
Fakruddin, M., Rahaman, M., Ahmed, M., & Hoque, M. (2014). Occurrence of Enterobacteriaceae with Serological Cross Reactivity towards Salmonella spp., Shigella spp. and Vibrio cholerae in Food. Microbiology Research Journal International, 5(1), 44-51. https://doi.org/10.9734/BMRJ/2015/11896
Section
Original Research Article