Bio-activities of Tetracycline and Algae Food Supplement Algo-Bio® on Escherichia coli Antimicrobial Resistance Isolated from Piglet’s Intestinal Flora
Amine Naty Tadiogo Kone *
Laboratory of Biotechnology and Microbiology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire.
Baguy Mohamed Ouattara
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
Bertin Tiekoura
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
Fernique Kouadio Konan
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
Eugène Koffi
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
Innocent Kouamé Kouadio
Laboratory of Biotechnology and Microbiology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire.
Adjéhi Dadie
Laboratory of Biotechnology and Microbiology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire.
Nathalie Kouadio Guessennd
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire and Laboratory of Bacteriology-Virology, Department of Medical Sciences, Félix Houphouet Boigny University, Abidjan, Côte d'Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Aims: This study aimed at evaluating the effect of Algo-Bio® use on Escherichia coli resistance strains isolated from piglets intestinal flora.
Study Design: Bacteriological study.
Place and Duration of Study: Laboratory of the National Reference Center for antibiotics at Institute Pasteur Côte d’Ivoire, between March 2018 and June 2018.
Methodology: A breeding of three batches of two piglets was carried out, then treatments with tetracycline and Algo-Bio® were administered to them. Enterobacteria was isolated on Mac Conkey medium added up with tetracycline and resistance rates were determined. Escherichia coli resistant strains have been identified and antibiotic susceptibility test was performed using disk diffusion method on Müller-Hinton agar.
Results: Enterobacteria resistance rates increased ranging from 18.4% (D0) to 81.5% (D4) to tetracycline-treated piglets and respectively from 25.7% (D0) to 29% (D4) and from 22.3% (D0) to 24.5% (D4), in control piglets and those treated with Algo-Bio®. Antibiotic susceptibility test of Escherichia coli strains isolated from piglets treated with tetracycline showed high resistance to ceftazidime (83.3%), amoxicillin (76.9%) and tetracycline (92.3%) with 39.4% strains producing ESBL, 23.7% producing PHL and 5.2% of producing CHP on D4. Escherichia coli strains isolated from control piglets and Algo-Bio® treated piglets revealed a decrease of ESBL respectively from 17.6% (D0) to 13.7% (D4) and from 12.5% (D0) to 6.4% (D4).
Conclusion: The study showed that the use of Algo-Bio® does not induce an evolution of antimicrobial resistance in Microbiota strains and consequently this dietary supplement can be used as a good alternative to antibiotics.
Keywords: Antibioresistance, Algo-Bio®, piglets, Escherichia coli, tetracycline, phenotype.