Impact of Diameter of Inhibition in Crowded Plates on the in vitro Inhibitory Strength of Potential Antibiotic-Producing Bacteria Isolated from Some Natural Habitats
Olasinbo Olumuyiwa Balogun
*
Department of Clinical Laboratory Services, Institute of Human Virology, Nigeria, IHVN Campus, Plot 62, Emeritus Umaru Shehu Avenue, Cadastral Zone COO, After Baze University Abuja, FCT, Nigeria.
Sylvanus Chukwudi Ugoh
Department of Microbiology, University of Abuja, Abuja, Nigeria.
Olabisi Peter Abioye
Department of Microbiology, Federal University of Technology, P.M.B. 65, Minna, Nigeria.
Peters Oluwale Oladosu
Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research and Development (NIPRD), Abuja, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Antimicrobial resistance is a global problem, and efforts to replace failing antibiotics are crucial. We investigated the impact of the diameter of inhibition (mm) in crowded plates of potential antibiotic-producing bacteria on their in-vitro inhibitory strength. Our previous research identified 12 potential antibiotic-producing bacteria from natural habitats and obtained their diameters of inhibition in preliminary crowded plates. The potential candidates were further challenged with some pathogenic microbes by the Agar-well diffusion technique to test their strength. The results showed that the diameter of inhibition of the potential antibiotic-producing isolates obtained at the crowded plate technique level was not a predictor of the potency of potential antibiotic-producing bacteria (rs = 0.52, P = 0.08). In summary, the selection of potent candidate microbes for the development of new antimicrobial compounds against multidrug-resistant pathogens cannot be determined based on the diameter of inhibition in a preliminary investigation using the crowded plate technique. Further studies involving complementary techniques are necessary to determine the best potential candidate organisms for the development of novel antimicrobial compounds.
Keywords: Crowded plate technique, antibiotic-producing bacteria, diameter of inhibition, agar-well diffusion method, natural habitats