Detection of ESBLs in Pseudomonas aeruginosa Isolated from Different Body Fluids
Javid Ahmad Bhat *
Department of Microbiology, Government Medical College Anantnag, India.
Abiroo Jan
Department of Microbiology, Government Medical College, Anantnag, India.
Rubeena Hakak
Department of Microbiology, Government Medical College Anantnag, India.
*Author to whom correspondence should be addressed.
Abstract
Background: The present study was carried out in order to detect the production of ESBLS in Pseudomonas aeruginosa and their susceptibility pattern against different antibiotics.
Materials and Methods: A total of 100 isolates of P. aeruginosa was analyzed for the production of ESBLs by using phenotypic confirmatory test and the results were determined. The antibiotic susceptibility against various antibiotics was performed by the Kirby-Bauer Disc diffusion method on Mueller-Hinton Agar.
Results: Out of the 100 isolates tested 20 isolates were positive for the production of ESBLs, with a high prevalence of 36.3% found in the pus samples followed by the sputum (26%), blood (22.2%) and urine (5.4%), respectively. Imipenem was found to be the most effective antibiotic with 100 per cent susceptibility followed by Ofloxacin. A drug resistance of 57% for ceftazidime was noted against P. aeruginosa followed by cefotaxime. A high prevalence rate of P. aeruginosa was detected in males.
Conclusion: This study showed the rising tendency of multidrug-resistant strains of the Enterobacteriaceae family, especially P. aeruginosa in the hospital settings, which is a great concern for clinicians and patients to treat these resistant strains.
Keywords: P. aeruginosa, drug resistance, ESBLs, β-lactams