In vitro Growth of Trichophyton Rubrum in the Presence of 80% Ethanolic Extract of Grapefruit Pepins as Antifungal Agent
Ouattara S *
Biology and Health Laboratory, Felix Houphouet-Boigny University, Cocody, Abidjan, Ivory Coast, 22 BP 582 Abidjan 22, Côte d'Ivoire.
Yao K. L.
Biology and Health Laboratory, Felix Houphouet-Boigny University, Cocody, Abidjan, Ivory Coast, 22 BP 582 Abidjan 22, Côte d'Ivoire.
Thes P. M. Épse Soumahoro
Biology and Health Laboratory, Felix Houphouet-Boigny University, Cocody, Abidjan, Ivory Coast, 22 BP 582 Abidjan 22, Côte d'Ivoire.
Yapo A. F
Biology and Health Laboratory, Felix Houphouet-Boigny University, Cocody, Abidjan, Ivory Coast, 22 BP 582 Abidjan 22, Côte d'Ivoire.
Yapi A
Biology and Health Laboratory, Felix Houphouet-Boigny University, Cocody, Abidjan, Ivory Coast, 22 BP 582 Abidjan 22, Côte d'Ivoire.
Zirihi G. N
Botany Laboratory, Felix Houphouet-Boigny University, Cocody, Abidjan, Ivory Coast, 22 BP 582 Abidjan 22, Côte d'Ivoire.
Djaman A. J.
Biology and Health Laboratory, Felix Houphouet-Boigny University, Cocody, Abidjan, Ivory Coast, 22 BP 582 Abidjan 22, Côte d'Ivoire and Medical and Fundamental Biochemistry, Pasteur Institute / Company, Cocody, Abidjan, Ivory Coast, 01 BP 490 Abidjan 01, Côte d'Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Many modern antifungal drugs have limits in terms of germ resistance, and to remedy this, plant compounds are being explored to find new more effective principles.
Aim: With the aim of complementing the efforts of modern medicine against viral, bacterial, parasitic and fungal diseases, the plant extract of 80% hydroalcoholic grapefruit seed extract of Citrus paradisi was tested on the in vitro growth of Trichophyton rubrum.
Methods: Antifungal tests were carried out on Sabouraud medium, to which the plant extracts were incorporated using the double dilution method in inclined tubes. A 10 µL volume of the Trichophyton rubrum suspension was inoculated on the culture medium contained in the test tubes.
Results: Results showed that Trichophyton rubrum was sensitive to the 80% hydroethanolic extract of Citrus paradisi in a dose-dependent manner.
Conclusion: The hydroethanolic extract may be a source for the development of Traditional Improved Medicines (TIM) against skin mycosis.
Keywords: Citrus paradisi, 80% hydroethanolic extract, mycosis, Trichophyton rubrum, grapefruit seed