Isolation and Identification of Bacteria from Microplastic-Polluted Soil from Three Geopolitical Zones in Osun State, Nigeria
Oladipupo, O.A. *
Department of Science Laboratory Technology, Osun State Polytechnic, Iree, Osun State, Nigeria.
Sindiku, O.
Department of Biological Science, Lead City University, Ibadan, Oyo State, Nigeria.
Olowe, B.M.
Department of Biological Science (Microbiology Unit), School of Life Sciences, Bamidele Olumilua University of Education, Science and Technology, P.M.B 250, Ekiti State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Plastic pollution has become a pressing environmental concern due to its widespread presence and persistence in terrestrial ecosystems. This study isolated and identified the microplastic-degrading bacteria from plastic-polluted soils in Osun State, Nigeria. Soil samples were collected from six locations in three zones, and their physicochemical properties were analyzed. Microbial isolation and identification were carried out using morphological and biochemical tests standards. The pH ranged from 8.2 to 8.8. and the temperature ranged from 25°C to 29.1°C. Microbiological assessments revealed high bacteria counts, with total viable bacterial counts ranging from 2.5 x 106 to 4.0 x 106 CFU/g. Four bacterial strains were isolated: Bacillus subtilis, Bacillus licheniformis, Bacillus amyloliticus, and Streptococcus spp. The isolated bacteria can be further studied for their enzymatic activities, offering eco-friendly solutions for plastic degradation. Further research and policy development are recommended to support bioremediation efforts in Nigeria and other regions affected by plastic pollution.
Keywords: Bacteria, microplastic, pollution, soil