Biogenic Synthesis of Silver Nanoparticles by Native Isolate of Trichoderma viride (NRT -1) and Its Effect on Rhizoctonia solani Kuhn Causing Sheath Blight of Rice
Y. Sandhya *
Department of Plant Pathology, S. V. Agricultural College, Tirupati (Dt), PIN: 517 502, India.
C.P.D. Rajan
Department of Plant Pathology, ARS, ANGRAU, Nellore, India.
M. Reddi Kumar
Department of Plant Pathology, ANGRAU, Tirupati, India.
*Author to whom correspondence should be addressed.
Abstract
A total of 10 Trichoderma isolates were obtained from 10 rhizosphere samples The antagonistic effects of ten isolates of Trichoderma spp. were assessed based on their ability to inhibit the Rhizotonia solani by dual culture technique. Among the ten Trichoderma isolates tested, NRT-1 isolate showed maximum percentage of inhibition (68.5%). It was observed that NRT-1 showed overgrowth of pathogen by 0.2cm and was most effective compared to all other test isolates of Trichoderma. Among the three concentrations, tested on mycelial growth the highest inhibition (88.19%) was observed at 125 ppm followed by 100 ppm (52.93 Among the four tested concentrations on sclerotial viability (50, 75, 100 and 125 ppm) the highest per cent inhibition was observed at 125 ppm (85%) followed by 100 ppm (47.50%).
Keywords: Bio-nano silver particles, sclerotia, Rhizoctonia solani, sheath blight