In-vitro Evaluation of Soybean Nodules Associated Bacteria for Their Plant Growth Promoting Ability on Agricultural Soils in Center of Côte d’Ivoire
Edouard Loukou KOUADIO
UFR Agro-forestry, Department of Agropedology, Laboratory of Agricultural Production Improvement, Jean Lorougnon Guede University, B.P: 150 Daloa, Côte d’Ivoire.
Raïssa Kaéda Nassé GUEI
*
UFR Agro-forestry, Department of Agropedology, Laboratory of Agricultural Production Improvement, Jean Lorougnon Guede University, B.P: 150 Daloa, Côte d’Ivoire.
Kouadio AMANI
Central Laboratory of Soils, Water and Plants, National Center for Agronomic Research (CNRA), 01 BP 633 Bouaké 01, Côte d’Ivoire.
Adama OUATTARA
Rice Program, Research Station of Man, National Center for Agronomic Research (CNRA), BP 440 Man, Côte d’Ivoire.
Athanase Kra KOUASSI
UFR Agro-forestry, Department of Biochemistry and Microbiology, Laboratory of Agrovalorization, Jean Lorougnon Guede University, B.P: 150 Daloa, Côte d’Ivoire.
Mathurin KOFFI
UFR Environment, Laboratory of Biodiversity and Sustainable Management of Tropical Ecosystems, Research Unit in Genetics and Molecular Epidemiology (URGEM), Jean Lorougnon Guede University, B.P: 150 Daloa, Côte d’Ivoire.
Abdelkarim FILALI-MALTOUF
Laboratory of Microbiology and Molecular Biology, Faculty of Sciences, University Mohammed V in Rabat, Morocco.
Ibrahim KONATE
UFR Agro-forestry, Department of Biochemistry and Microbiology, Laboratory of Agrovalorization, Jean Lorougnon Guede University, B.P: 150 Daloa, Côte d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
The overuse of chemical fertilizers poses significant risks to preserving the environment, food safety and human health. As sustainable alternatives, plant growth-promoting rhizobacteria offer a ecological approach to improving plant productivity and soil fertility. This study aims to assess the plant growth promoting (PGP) traits of 10 bacterial isolates derived from soybean nodule in order to select the most efficient ones and use them as biofertilizers. These bacterial isolates were tested for their PGP characteristics such as AIA and ammonia production, phosphate, potassium and calcium solubilization.
AS results, 60% of isolates produced IAA, 100% ammonia, 60% solubilized phosphate and 40% solubilized potassium and calcium. Two (02) isolates (RSC323, RSC325) showed several characteristics of plant growth promoting. But, one isolate (RSC323) was fund to be most efficient. It shown the higest solubilization indices, PSI (381.48±16.97%), KSI (211.11±19.24 %) and CaSI (220.00±40.00%) and produced more IAA and Ammonia.
These results suggest that the selected rhizobacterial strains have significant potential as biofertilizers to support sustainable agriculture in tropical ecosystems.
Future work should focus on the identification of 16S rRNA genes and field trials to confirm their effectiveness in promoting plant growth under natural conditions.
Keywords: PGPR, biofertilizers, bacteria, soybean, in-vitro