Prevalence and Phenotypic Characterization of Extended-Spectrum β- Lactamase (ESBL)-Producing Uropathogenic Enterobacteriaceae Isolated at the Fann University Hospital
Aïssatou Ahmet NIANG *
Bacteriology-Virology Laboratory, National University Center of Fann, Dakar, Senegal and Bacteriology-Virology Laboratory, Faculty of Medecine, Pharmacy and Odontology, Cheikh Anta DIOP University of Dakar, Senegal.
Amadou DIOP
Bacteriology-Virology Laboratory, Faculty of Medecine, Pharmacy and Odontology, Cheikh Anta DIOP University of Dakar, Senegal and Bacteriology-Virology laboratory of Albert Royer National Children’s Hospital Center, Dakar, Senegal.
Baidy DIEYE
Bacteriology-Virology Laboratory, Faculty of Medecine, Pharmacy and Odontology, Cheikh Anta DIOP University of Dakar, Senegal and Bacteriology-Virology laboratory of Albert Royer National Children’s Hospital Center, Dakar, Senegal.
Fatoumata DIALLO
Bacteriology-Virology Laboratory, National University Center of Fann, Dakar, Senegal and Bacteriology-Virology Laboratory, Faculty of Medecine, Pharmacy and Odontology, Cheikh Anta DIOP University of Dakar, Senegal.
Habibou SARR
UFR in Health Sciences, University of Ziguinchor, Senegal.
Madiagne DER
Bacteriology-Virology Laboratory, National University Center of Fann, Dakar, Senegal.
Niokhor Ngobe SENE
Bacteriology-Virology Laboratory, National University Center of Fann, Dakar, Senegal.
Papa Lat Coumba SECK
Direction of Laboratories, MoH, Dakar, Senegal.
Pape Daouda CISSE
Direction of Laboratories, MoH, Dakar, Senegal.
Aminata Jeanette BADJI
Bacteriology-Virology Laboratory, Faculty of Medecine, Pharmacy and Odontology, Cheikh Anta DIOP University of Dakar, Senegal.
Sekhou Cisse
Bacteriology-Virology Laboratory, Faculty of Medecine, Pharmacy and Odontology, Cheikh Anta DIOP University of Dakar, Senegal.
Roughyatou KA
UFR in Health Sciences, University of Thies, Senegal.
Mouhamadou Lamine DIA
Bacteriology-Virology Laboratory, National University Center of Fann, Dakar, Senegal and Bacteriology-Virology Laboratory, Faculty of Medecine, Pharmacy and Odontology, Cheikh Anta DIOP University of Dakar, Senegal.
Seynabou LO
UFR in Health Sciences, University of Gaston Berger of Saint Louis, Senegal.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Urinary tract infections caused by extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-producing Enterobacteriaceae) are a public health problem in the world. This study aims to determine the prevalence and resistance profiles of ESBL-producing Enterobacteriaceae isolated at the Fann University Hospital Center.
Methods: This is a prospective study of urine samples analyzed at the bacteriology laboratory of the Fann University Hospital Center. Bacterial identification and antibiograms were performed according to CA-SFM recommendations.
During the study, 2,852 urine samples were collected from patients who came for consultation or were hospitalized in the study facility.
Results: Of all the enterobacteria isolated from 20% of urine samples, 32% were ESBL-producing. ESBL-producing E. coli were mainly isolated from outpatients (71%) and elderly subjects (≥ 60 years: 44%). The main co-resistances concerned gentamicin (66%), cotrimoxazole (88%), and fluoroquinolones (75%). Good sensitivity to imipenem (80%) and chloramphenicol (80%) was observed.
Conclusion: These results confirm the high community prevalence of ESBL-producing E. coli in Dakar and the threat of multidrug resistance. The continued sensitivity to carbapenems should encourage ongoing surveillance and prudent use of antibiotics in Senegal.
Keywords: Enterobacteriaceae, phenotypic, ESBL, CHNU fann