Study of Hepatitis E Virus in Minia Governorate, Upper Egypt
Mona Abdel-Monem Esmail
Department of Microbiology and Immunology, Minya University, Egypt.
Sahr Mohammed Abo EL Euoon
Department of Microbiology and Immunology, Minya University, Egypt.
Mohammed Sayed Mohammed *
Department of Microbiology and Immunology, Minya University, Egypt.
Shimaa Abd Elbadie Ali Hasanin
Department of Microbiology and Immunology, Faculty of Dentist, El Nahda University, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Aim: Hepatitis E is a viral liver disease caused by the hepatitis E virus (HEV), which is endemic in many tropical countries. We aimed to study the incidence of HEV infection at Minia governorate through Enzyme Linked Immunosorbent Assay (ELISA) and Polymerase Chain reaction (PCR) tests.
Methodology: The study found that among 129 cases of acute hepatitis, laboratory methods for diagnosis of Hepatitis viruses(A , B and c) were done by using ELISA for detection HBs Ag, HCV antibodies and HAV antibodies and all positive cases of Hepatitis A, B and C were excluded. All Negative cases were screened for HEV Using ELIZA and PCR
Results: Eight cases were positive by PCR and Five cases positive by ELIZA.
Conclusion: The incidence of HEV was low and PCR method was accurate than ELIZA in diagnosis.
Keywords: Acute hepatitis, HEV infection, ELIZA and PCR