Bacteriological Investigation of E. coli in Urinary Tract Infection in South Port Said City
Ahmed M. Abdelaty *
Department of Botany, Faculty of Science, Port Said University, Egypt.
Mahmoud M. Zaki
Department of Botany, Faculty of Science, Port Said University, Egypt.
Amro S. Hanora
Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal University, Egypt.
Magdy Bahgat
Department of Microbiology, Faculty of Science, Port Said Univesity, Egypt.
*Author to whom correspondence should be addressed.
Abstract
UTI is characterized by the evidence of uropathogens and pyuria and is accompanied by various clinical manifestations depending on the area of involvement.
Aim: The aim of this study was to isolate the bacterial infection to urinary tract infection in men and women at different ages and to investigate the susceptibility of bacteria implicated in urinary tract infection to different antimicrobial drugs (antibiotics). Also to determine minimum inhibitor concentration for resistant bacteria to antibiotic and molecular characterization of E. coli associated.
Materials and Methods: One hundred urine specimens were received from patients admitted during January till December 2015. E. coli isolates were confirmed by typical colonial morphology, and identified by differential tests as well as by the growth on characteristic agar, Susceptibility testing was carried out by disk diffusion method. Among the 100 isolates, 72 bacterial strain was isolated from the urine specimens of infected Patient admitted at Port-said area out-patient clinics, using agar medium and macCkonkey agar, This organism was characterized by biochemical tests and showed similarity with E. coli. The genomic level confirmation done with 16S rDNA primer by submitting the genomic sequence to Gene Bank under acc.No-GU046545 after comparing, showed 98% sequence similarity with E. coli. Antibiotic susceptibility test revealed that imipenem, Amikacin, Nitrofurantoin and gentamicin are the lowest resistant rate with percent of 79.2, 76.4, 75 and 61% respectively and ampicillin showed the highest resistant rate with 89%. Pattern on antibiotic susceptibility test showed high resistant rate to some antibiotic that made it difficult for pregnant patients, although its frequency was low. The most prevalent bacterial pathogen in Port-saed city was E. coli and the most sensitive antibiotic against it is imipenem.
Keywords: Antibiotic sensitivity, molecular characterization, minimum inhibitor concentration