Microbiology Research Journal International

Aims: This study was conducted for isolation, identification and probiotic profiling of Lactobacillus spp. from the intestine of indigenous and non-indigenous Channa punctata species.

Study Design: Lactobacillus spp. from the intestine of Channa punctata fish was isolated and cultured as well as screened for probiotic properties and profiling.

Place and Duration of Study: The research work was carried out in Biotechnology Laboratory, Department of Genetic Engineering and Biotechnology, Jessore University of Science and Technology, Jessore-7408, Bangladesh, from July to October, 2015.

Methodology: Lactic Acid Bacteria (Lactobacillus spp.) were isolated from the intestine of dissected Channa punctata by using MRS agar medium following spread plate method. Isolated bacteria were subjected to gram staining, microscopic observation and biochemical tests example catalase for identification of Lactobacillus spp. Thereafter, bile salt tolerance test, phenol tolerance test and antibiotic sensitivity test were done for probiotic profiling.

Results: Two Lactic Acid Bacteria were isolated from indigenous and non-indigenous Channa punctata species where total bacterial cell was counted as 2.1×10¹⁰ and 1.9×10⁹ cfu/gm. Both the fish intestines contain bacteria having gram positive, non-sporulating, catalase negative rods which were confirmed to be Lactobacillus. They were able to survive in bile salt and phenol at 0.1, 0.2, and 0.3% concentration.  The Lactobacillus isolated from indigenous Channa punctata was resistant to Azithromycin, Cefuroxime, Ciprofloxacin, Tetracycline, Ampicillin, Erythromycin, Vancomycin, Chloramphenicol and Co-trimoxazole while Lactobacillus from non-indigenous C. punctata showed almost same resistant except Cefotaxime and Gatifloxacin. They are claimed as probiotics.

Conclusion: Thus, the study claimed that the isolated, counted and identified Lactobacillus spp. are belong to probiotic that can exert beneficial health action for human.