Screening, Growth Characterization and Alkaline Phosphatase Potential of Pseudomonas plecoglossicida from Mangrove Soil

S. Sankaralingam *

Saraswathi Narayanan College, Madurai, Tamilnadu, India.

B. Harinathan

Saraswathi Narayanan College, Madurai, Tamilnadu, India.

S. Palpperuma

Saraswathi Narayanan College, Madurai, Tamilnadu, India.

D. Kathiresan

Saraswathi Narayanan College, Madurai, Tamilnadu, India.

S. Rajendran

Saraswathi Narayanan College, Madurai, Tamilnadu, India.

T. Sivakumar

Ayya Nadar Janaki Ammal College, Sivakasi, Tamilnadu, India.

T. Shankar

Ayya Nadar Janaki Ammal College, Sivakasi, Tamilnadu, India.

G. Prabakaran

Ayya Nadar Janaki Ammal College, Sivakasi, Tamilnadu, India.

N. Sivakumar

Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, India.

*Author to whom correspondence should be addressed.


Abstract

The study was carried out to isolate phosphate solubilizing bacteria from mangrove soil collected from the Manakudi estuary, Kanyakumari, Tamilnadu, India. Around 20 colonies were obtained in Zobell marine agar plates screened on Pikovskaya’s agar plate for determining their phosphate solubilizing ability. Out of these 20 isolates, one isolate exhibited better phosphate solubilizing ability. Identification of strain was carried out and confirmed by cultural, biochemical and 16S rDNA sequences. The potent isolate was identified as Pseudomonas plecoglossicida. Various factors were tested for pH, temperature, carbon and nitrogen sources, tricalcium phosphate influenced bacterial growth and phosphate activity in static and shaking conditions and thus characterized.

Keywords: Pseudomonas plecoglossicida, phosphate solubilizing bacteria, 16S rDNA sequencing


How to Cite

Sankaralingam, S., B. Harinathan, S. Palpperuma, D. Kathiresan, S. Rajendran, T. Sivakumar, T. Shankar, G. Prabakaran, and N. Sivakumar. 2016. “Screening, Growth Characterization and Alkaline Phosphatase Potential of Pseudomonas Plecoglossicida from Mangrove Soil”. Microbiology Research Journal International 17 (6):1-11. https://doi.org/10.9734/BMRJ/2016/28488.

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