In vitro Antibacterial and Antitubercular Activities of Leaf Extracts of Senna occidentalis

Main Article Content

K. T. Olatunji
Y. Ya’aba
S. B. Mohammed
I. J. Akah
O. C. Daniel
P. O. Oladosu


Aim: This study aimed to evaluate the antibacterial and antitubercular activities of ethylacetate and ethanol leaf extracts of Senna occidentalis.

Study Design: Fresh leaves of Senna occidentalis collected from Suleja, Niger state were used for this study against some medically important micro-organisms viz; Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella paratyphi, Klebsiella pneumoniae, Mycobacterium bovis and Mycobacterium smegmatis.

Place and Duration of Study: The study was conducted in Abuja, Nigeria at the Department of Microbiology and Biotechnology, National Institute for Pharmaceutical Research and Development [NIPRD], from February 2019 to April 2019.

Methodology: Senna occidentalis leaves were extracted successively with ethyl-acetate and ethanol. The obtained extracts were tested in vitro for antibacterial activity by agar well diffusion method, while anti-tubercular screening was carried out by broth micro-dilution method. A fixed-dose concentration of chloramphenicol was used as a control drug against the bacterial isolates while isoniazid was used as control drug against the mycobacterium isolates.

Results: The in vitro antibacterial screening showed that the crude extracts exhibited varying activity against the different microbes with highest zone of inhibition at 12 mm, and anti-tubercular activity with MICs ranging from 97.6-390.6 μg/mL.  Among these extracts, ethyl-acetate extract showed significant antibacterial activity against most of the test micro-organisms. The most susceptible micro-organism was P. aeruginosa (12mm zone in ethyl-acetate at 80 mg/mL) followed by B. subtilis (10 mm zone in ethyl-acetate extract at 80 mg/mL) and E. coli (9 mm zone in ethyl-acetate extract at 80 mg/mL). The ethanol extract was the most effective in inhibiting the growth of M. smegmatis and M. bovis with MICs of 97.6 μg/mL and 195.3 μg/mL.

Conclusion: The activities observed could be attributed to the presence of some active metabolites contained in the extracts which could be useful in drug development for therapeutic purposes.

Senna occidentalis, anti-bacterial activity, anti-tubercular activity, micro-organisms.

Article Details

How to Cite
Olatunji, K. T., Ya’aba, Y., Mohammed, S. B., Akah, I. J., Daniel, O. C., & Oladosu, P. O. (2019). In vitro Antibacterial and Antitubercular Activities of Leaf Extracts of Senna occidentalis. Microbiology Research Journal International, 28(3), 1-8.
Original Research Article


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