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Aim: This research was designed to assess the molecular identities and antibiotic sensitivity pattern of the bacteria isolated from decomposed domestic food wastes in Akure metropolis.
Methodology: Fifteen bacteria were obtained from the Department of Microbiology, Federal University of Technology Akure. The DNA molecules of the bacterial isolates were extracted using bacterial DNA Mini-Prep Kit. The DNA extracted was amplified and sequenced using universal bacterial primers and ABI Prism DNA sequencer respectively prior their nucleotides blast. The antibiotic sensitivity test of the bacterial isolates was carried out using plate assay.
Results: The identified bacterial isolates including Bacillus megaterium, Clostridium difficile, B. thuringiensis, B. sphaericus, B. mycoides, B. cereus, Pseudomonas asplenii, Paenibacillus macerans, Lactobacillus jensenii, B. badius, B. licheniformis, B. subtilis and L. delbrueckii retained their original name after the molecular identification with the exception of C. humiferium and B. pumilus that changed to C.pseudotuberculosis and B. cereus respectively while five bacteria showed no result at the sequencing stage. The molecular techniques revealed the strain name of the bacteria. L. delbrueckii was resistant to amoxicillin, chloramphenicol, cefriazone while susceptible to erythromycin with a zone of inhibition (59.77±0.66 mm). C. difficile was susceptible to gentamycin with a zone of inhibition (13.93±0.47 mm).
Conclusion: From this study, the bacterial isolates (C. pseudotuberculosis, B. cereus, B. licheniformis, B. subtilis, and B. cereus) demonstrated multidrug resistant property. Therefore, this could constitute a serious health threat to the people living in the environment where the wastes are dumped indiscriminately.
Retraction Notice: This paper has been retracted from the journal. This journal is determined to promote integrity in research publication. This retraction is in spirit of the same. After formal procedures editor(s) and publisher have retracted this paper on 07th November 2019. Related policy is available here: http://goo.gl/lI77Nn