Biopotential of Some Trichoderma spp. against Growth of Fusarium oxysporum f. sp. Vasinfectum Causal Agent of Cotton Wilt
Kouakou Tanoh Hilaire *
Laboratoire de Biologie et Amélioration des Productions végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Bolou-Bi Bolou Antoine
Laboratoire de Physiologie Végétale, UFR Biosciences, Université Félix Houphouët Boigny, 22 BP 582 Abidjan 22, Côte d’Ivoire.
Soko Dago Faustin
Laboratoire de Physiologie Végétale, UFR Agroforesterie, Université Jean Lorougnon Guédé, BP 150 Daloa, Côte d’Ivoire.
Coulibaly Souleymane
Laboratoire de Biologie et Amélioration des Productions végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Kouakou Kouakou Laurent
Laboratoire de Biologie et Amélioration des Productions végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Koné Tchoa
Laboratoire de Biologie et Amélioration des Productions végétales, UFR des Sciences de la Nature, Université Nangui Abrogoua, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Koné Daouda
Laboratoire de Physiologie Végétale, UFR Biosciences, Université Félix Houphouët Boigny, 22 BP 582 Abidjan 22, Côte d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Evaluation of the antagonistic activity of some Trichoderma species by direct confrontation method was used in this study. The mycoparasitism inhibitory effects of Trichoderma species on the growth of the causal agent of cotton Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) were investigated by dual culture under in vitro condition. Seventeen isolates of Trichoderma and one strain of Fusarium oxysporumf. sp. vasinfectum (FOV) were used. These different isolates of Trichoderma were in vitro confronted to FOV. The invasion ability of antagonist colonies was measured during 14 days. Thus, at the end of these control trials, isolates of Trichoderma have controlled the growth of FOV. Indeed, FOV is confined to a small area and is unable to extend its growth after the third day of incubation in the culture room at 30°C. Beyond this period and after then days, they never sporulated, revealing the inhibitory action of Trichoderma against FOV. Consequently, the Trichoderma isolates were found able to biologically control of FOV growth.
Keywords: Cotton, biological control, Fusarium wilt, mycoparasitism, Trichoderma spp