Detection of TEM and SHV Genes in Clinical Escherichia coli and Klebsiella pneumoniae Strains ESBL Isolated in Neonatology and Pediatric Units
V. M’bengue Gbonon *
Laboratory of Bacteriology-Virology, Unit of Antibiotics, Pasteur Institute of Côte d'Ivoire, Côte d'Ivoire.
S. A. Afran
Laboratory of Genetics and Species Improvement, UFR Biosciences, Felix Houphouët Boigny University of Abidjan, Côte d'Ivoire.
K. N. Guessennd
Laboratory of Bacteriology-Virology, Unit of Antibiotics, Pasteur Institute of Côte d'Ivoire, Côte d'Ivoire.
A. A. Toty
Laboratory of Bacteriology-Virology, Unit of Antibiotics, Pasteur Institute of Côte d'Ivoire, Côte d'Ivoire.
T. F. B. Diplo
Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouët Boigny University of Abidjan, Côte d'Ivoire.
A. S. P. N’Guetta
Laboratory of Genetics and Species Improvement, UFR Biosciences, Felix Houphouët Boigny University of Abidjan, Côte d'Ivoire.
M. Dosso
Laboratory of Bacteriology-Virology, Unit of Antibiotics, Pasteur Institute of Côte d'Ivoire, Côte d'Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Aims: To detect blaTEM and blaSHV genes in Escherichia coli (E. coli) and Klebsiella pneumoniae (K. pneumoniae) strains resistant to β-lactams isolated from neonatal and pediatric infections.
Place and Duration: Bacteriology-Virology Department and Molecular Biology platform of Pasteur Institute of Côte d'Ivoire from January 2012 to November 2015.
Methods: A total of 38 strains of E. coli and K. pneumoniae ESBL isolated and identified according to classical bacteriology techniques from neonatal and pediatric infections, were subject of our study. Search for blaTEM, and blaSHV genes were carried out by conventional PCR.
Results: Molecular research showed that 52.6% of strains were carrying blaTEM gene and blaSHV gene was present in 36.9%. blaTEM and blaSHV genes were present simultaneously in 36.9% of strains.
Conclusion: This study revealed a predominance of blaTEM genes in E. coli and K. pneumoniae ESBL strains.
Keywords: blaTEM, blaSHV genes, E. coli, K. pneumoniae, neonatal and pediatric infections