Characterization of the Genetic Supports for Betalactam Resistance in Escherichia coli Strains of Porcine Origin Producing Extended-spectrum Beta-Lactamase (ESBL)
I. K. Kouadio *
Laboratory of Microbiology and Molecular Biology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire and Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
N. Guessennd
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire and Laboratory of Bacteriology-Virology, Department of Medical Sciences, Félix Houphouet - Boigny University, Abidjan, Côte d'Ivoire.
A. Dadié
Laboratory of Microbiology and Molecular Biology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire.
A. Konaté
Laboratory of Molecular Biology, Epidemiology and Monitoring of Foodborne Bacteria and Viruses, Ouaga I Professor Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
V. Gbonon
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
B. Tiékoura
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
S. Kpoda
Laboratory of Applied and Nutritional Sciences, Ouaga I Professor Joseph KI-ZERBO University, Ouagadougou, Burkina Faso.
M. B. Ouattara
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
F. Konan
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire.
A. Ajayi
Department of Microbiology, University of Lagos, Nigeria.
M. Djè
Laboratory of Microbiology and Molecular Biology, Department of Food Science and Technology, Nangui Abrogoua University, Abidjan, Côte d'Ivoire.
M. Dosso
Department of Bacteriology-Virology, National Reference Center for Antibiotics, Pasteur Institute of Côte d'Ivoire, Abidjan, Côte d'Ivoire and Laboratory of Bacteriology-Virology, Department of Medical Sciences, Félix Houphouet - Boigny University, Abidjan, Côte d'Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Aims: The study was to evaluate the genetic potential of beta-lactam resistance in Escherichia coli strains producing extended-spectrum beta-lactamase (ESBL) isolated from the faecal flora of piglets.
Place and Duration of Study: National Reference Center for Antibiotics and Molecular Biology Platform of Pasteur Institute of Côte d'Ivoire, between June 2017 at July 2017.
Methodology: A detection of E. coli strains were carried out from 30 samples of faeces of piglets after five-day of treatment with amoxicillin was studied. The isolation of E. coli was performed on MacConkey agar supplemented with amoxicillin and identification using biochemical test. The Antibiotic susceptibility test was carried out according to diffusion method in agar medium.
Results: The genetic supports for betalactam resistance, in particular the bla TEM, bla SHV and bla CTX-Mgenes, have been detected by PCR in strains producing extended-spectrum beta-lactamase (ESBL). A total of thirty five (35) E. coli isolates (35%) showed ESBL production. The bla TEM, bla SHV and blaCTX-M genes were detected with frequency of 51%, 40% and 31% respectively. The strains were resistant to antibiotics from other families with the most common resistance profile consisting of tetracycline (100%), trimethoprim / sulfamethoxazole (80%), gentaminicin (70%), kanamycin (70%) and streptomycin (90%).
Conclusion: The detection of bla genes which are plasmid borne, therefore potentially horizontally transmissible to other strains, constitute a risk to public health and requires a monitoring of antibiotic-resistant bacteria in the animal production sector.
Keywords: Piglets, E. coli, ESBL, antibiotic, bla TEM genes, bla SHV, bla CTX-M