Detection of Salmonella spp. and Microbiological Analysis of Milk and Milk Based Products Available within Dhaka Metropolis, Bangladesh
Sharmina Yasmin
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Shahana Parveen
Institute of Food Science and Technology (IFST), Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh
Md. Sakil Munna
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
Rashed Noor *
Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka 1217, Bangladesh
*Author to whom correspondence should be addressed.
Abstract
Aims: With a previous observation of pathogenic drug-resistant microorganisms in raw milk samples, current investigation further endeavored to determine the presence of Salmonella spp. and portrayed a complete map of the overall microbiological feature of milk and milk based products available within Dhaka metropolis, Bangladesh.
Methodology: Conventional cultural and biochemical methods were carried out to isolate and enumerate the milk and milk based products accessing microorganisms. Standard agar disc diffusion method was conducted to assess the anti-bacterial resistance pattern of the isolated Salmonella spp.
Results: Out of 35 samples studied, 16 were found to be contaminated with E. coli (~106 cfu/ml) and 9 samples were found to be contaminated with Salmonella spp. (~105 cfu/ml). Fungal growth was also observed in all samples (~106). The Salmonella isolates were found to be resistant against ampicillin (10 µg), cotrimoxazole (25 µg), chloramphenicol (30 µg), ciprofloxacin (5 µg), cefixime (5 µg) and sensitive against ofloxacin (5 µg).
Conclusion: As projected from the current study, the proliferation of Salmonella spp. along with their drug-resistance traits could impart the morbidity and mortality associated risk to the overall public health safety.
Keywords: Milk, Pathogens, drug-resistance, Salmonella spp., food safety, public health