Isolation and Identification of Streptomyces rochei Strain Active against Phytopathogenic Fungi
Adil A. El Hussein *
Department of Botany, Faculty of Science, University of Khartoum, Sudan
Rihab E. M. Alhasan
Environmental and Natural Resources Research Institute, National Center for Research, Sudan
Suhair A. Abdelwahab
Biology Department, Duba University College, Tabuk University, Saudi Arabia
Marmar A. El Siddig
Department of Botany, Faculty of Science, University of Khartoum, Sudan
*Author to whom correspondence should be addressed.
Abstract
A total of 104 actinomycete isolates were recovered from farming soil samples collected from 11 states in Sudan. Upon screening for potential antifungal activity, an actinomycete isolate (R92) was found to be highly antagonistic against all of the tested phytopathogenic fungi. It was identified as Streptomyces rochei on the basis of its morphology, chemotaxonomy and 16S rDNA sequence analysis. In vivo antagonistic activities of the n-butanol extract of R92 culture were significant since the progress of Drechslera halodes leaf spot on sorghum and Alternaria alternata early blight on tomato was highly restricted and incidence of both diseases was greatly suppressed. Trials to evaluate the In vivo control efficacy of this extract under field conditions is recommended.
Keywords: Streptomyces rochei, In vivo activity, 16S rDNA, phytopathogens, Sudan