Concurrent Infection of Torque Teno Sus Virus and Porcine Circovirus Type 2 in a Clinical Case with Post-weaning Multisystemic Wasting Syndrome
Shao-Lun Zhai
Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China and Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China and College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
Jin-Xue Long
Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China and Department of R&D, Beijing CEVA-Huadu Biological Co., Ltd, Beijing 102600, China
Qin-Ling Chen
Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Man-Lin Luo
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China
Wen-Kang Wei
Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China
Shi-Shan Yuan *
Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China
*Author to whom correspondence should be addressed.
Abstract
Aims: The goal of this study was to identify possible concurrent infection of torque teno sus virus (TTSuV) and porcine circovirus type 2 (PCV2) in a clinical case with post-weaning multisystemic wasting syndrome (PMWS) on certain farm of Shanghai, China.
Place and Duration of Study: Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, between June 2009 and June 2010 & Institute of Animal Health, Guangdong Academy of Agricultural Sciences, between September and November, 2013.
Methodology: Multiply-primed rolling-circle amplification (MPRCA), a useful molecular tool, was performed to amplify genome sequence of TTSuV and PCV2. For serum sample of SH0822 from a clinical case with PMWS, the products of MPRCA were digested using EcoR I, Xba I, Sma I, Sac I, respectively. Moreover, Clustal W program (DNASTAR software) and MEGA 5.1 software (neighbour-joining method) was used to analysis its nucleotide homology and genetic relationship.
Results: Restriction digestion analysis showed one TTSuV genome-size fragment was presented in 1.2 % agarose gel, moreover, another PCV2 genome-size fragment was also presented. Nucleotide sequencing and phylogenetic analysis results suggested that its complete genome were 2823-nucleotide size and 1767-nucleotide size and they were divided into species TTSuV1b and genotype PCV2b, respectively.
Conclusion: Concurrent infection of TTSuV and PCV2 in a clinical case with PMWS was identified using MPRCA combining with restriction endonuclease digestion, which indicated that MPRCA was an effective tool to attain simultaneous detection and genome amplification of TTSuV and PCV2.
Keywords: Torque teno sus virus, porcine circovirus type 2, multiply-primed rolling-circle amplification, complete genome, genetic relationship