Optimization of Process Parameters for Improved Production of Bioactive Metabolites by Streptomyces tritolerans DAS 165T
Usha Kiranmayi Mangamuri
Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjunanagar, Guntur-522510, India.
Vijayalakshmi Muvva *
Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjunanagar, Guntur-522510, India.
Sudhakar Poda
Department of Biotechnology, Acharya Nagarjuna University, Nagarjunanagar, Guntur-522510, India.
Dayanand Agasar
Department of Studies and Research in Microbiology, Gulbarga University, Gulbarga-585106, India.
*Author to whom correspondence should be addressed.
Abstract
Aims: To optimize the process parameters for enhanced production of bioactive metabolites by Streptomyces tritolerans DAS 165T.
Place and Duration of Study: Department of Botany and Microbiology, April 2012 to August 2012.
Methodology: Agar well diffusion assay was employed to study the effect of environmental parameters such as incubation period, pH, temperature and salt concentration and influence of various nutrients such as carbon and nitrogen sources and minerals on the bioactive metabolite production by Streptomyces tritolerans DAS 165T.
Results: The production of antimicrobial metabolite was high when the strain was cultured for six days at 35ºC in medium (pH 7.5) with sucrose at the concentration of 2% (carbon source), soya peptone at the concentration of 1% (nitrogen source) and sodium chloride at the concentration of 5%.
Conclusion: This is the first report on the optimization of bioactive metabolite production by Streptomyces tritolerans DAS 165T. As the strain exhibited potent antimicrobial activity, it may be explored for biotechnological purposes.
Keywords: Optimization studies, streptomyces tritolerans, bioactive metabolites, environmental parameters, nutritional factors, antimicrobial activity.