Prevalence and Antibiotic Resistance Profile of Salmonella Isolates from Commercial Poultry and Poultry Farm-handlers in Jos, Plateau State, Nigeria
G. O. A. Agada *
National Veterinary Research Institute, Vom, Plateau State, Nigeria and Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
I. O. Abdullahi
Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria
M. Aminu
Department of Microbiology, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
M. Odugbo
National Veterinary Research Institute, Vom, Plateau State, Nigeria.
S. C. Chollom
National Veterinary Research Institute, Vom, Plateau State, Nigeria.
P. R. Kumbish
National Veterinary Research Institute, Vom, Plateau State, Nigeria.
A. E. J. Okwori
Department of Microbiology, Federal College of Veterinary and Medical Laboratory Technology, Vom, Plateau State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aim: This study was designed to investigate the prevalence and antibiotic resistance profile of Salmonella serovars from poultry and poultry farm-handlers.
Study Design: Investigative
Place and Duration of Study: Samples were analyzed at the Central Diagnostic Laboratory, National Veterinary Research Institute Vom and Department of Microbiology, Ahmadu Bello University, Zaria. This work was carried out between August 2012 and April 2013.
Methodology: Samples were pre-enriched in buffered peptone water followed by selective enrichment using Selenite Faeces Broth and Rappaport-Vassilidis Broth. Isolation and identification was made by inoculating the selectively enriched sample on to Salmonella-Shigella agar, Xylose Lysine Deoxycholate agar and Brilliant Green agar followed by confirmation of presumptive colonies using different biochemical tests and analytical profile index 20 E. Polyvalent (O) and (H) Salmonella antisera were used for serotyping the Salmonella isolates. The CLSI, 2010 method was used for antimicrobial susceptibility testing
Results: A prevalence rate of 10.9% was observed from the 450 samples. Serovars of Salmonella detected were S. Gallinarum 57.2%, S. Typhimurium 8.2%, S. Typhi 20.4%, S. Pullorum 6.1%, S. Enteritidis 6.1% and S. Paratyphi A 2.0%. Statistically, significant difference (p<0.05) was observed between isolates and occurrence at different sample sites. The isolates were 100% resistant to oxacillin, 96.0% to ampicillin, 93.9% tylosin, 83.7 5 ceftazidime and 63.3% oxytetracycline. Five of the isolates were 100% resistant to more than five different antibiotics. There was statistical significant difference (p<0.01) in antimicrobial resistance patterns exhibited by the serovars. However, the isolates showed sensitivity to gentamycin 100%, gendox 83.7%, ciprofloxacin 81.6% and amoxicillin-clavulanic acid 57.1%.
Conclusion: The study revealed emergence of multiple-drug resistant Salmonella serovars from poultry and poultry farm handlers. We therefore suggest further epidemiological studies.
Keywords: Poultry, Salmonella serovar, Nigeria, antibiotic resistance, farm handlers.