Molecular Detection of Giardia lamblia and Cryptosporidium parvum in Different Water Sources of District Bannu, Khyber Pakhtunkhwa, Province of Pakistan
Muhammad Shoaib Alam
Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Sana Ullah Khan
Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Sultan Ayaz
Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan
Noor ul Akbar
Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Muhammad Asim Khan
Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan
Iftikhar Ahmad
Department of Zoology, Kohat University of Science and Technology, Kohat 26000, Pakistan.
Muhammad Idrees
Division of Molecular Virology, CEMB, University of the Punjab Lahore-53700, Pakistan.
Muhammad Waqar *
Genome Center for Molecular Based Diagnostics and Research, Lahore, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
Background: Cryptosporidium parvum and Giardia lamblia are intestinal parasites that predominantly causes "waterborne" infections that are transmitted through consumption of contaminated water. Both parasites typically cause an acute short-term infections with self-limiting diarrhea as the main symptom in people with intact immune systems. However, in immunocompromised individuals, the symptoms are particularly severe and might be fatal.
Methods: The study was carried out in District Bannu Khyber Pukhtunkhwa, Pakistan for the detection of G. lamblia and C. parvum parasites in drinking water in different villages/localities (Kakki, Jamon Road, Kotka Juma Khan, Sokari, Mandan and Bannu City). Water samples n=75 were collected from different water sources between 1st August 2011 to 30th January 2012. These samples included tap, pond, borewell and hand pump water that were filtrated and residue was subjected to amplify by PCR.
Results: Overall prevalence of parasites was 36% (25/75), containing tap 17.64% (9/51) and pond water 75% (6/8), bore well water 41.66% (5/12) and hand pump water 50% (2/4). Similarly over all prevalence rate of tap water for C. parvum was 7.84% (4/51) while for G. lamblia was 9.80% (5/51) positive. The present study revealed that the people of the area should use the cleaned and filtered water.
Conclusion: Contamination of water with G. lamblia and C. parvum was found in water sources especially the drinking ones, of District Bannu which need proper water treatment to decontaminate and large scale studies are needed.
Keywords: G. lambli, C. parvum, diarrhea, water, PCR