Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum Subspecies Polymorphum in Nigeria
Francisca O. Nwaokorie *
Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria.
Kofoworola O. Savage
Department of Preventive Dentistry, College of Medicine of the University of Lagos, Nigeria
Muinah A. Fowora
Molecular Biology and Biotechnology Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria.
Patricia O. Ayanbadejo
Department of Preventive Dentistry, College of Medicine of the University of Lagos, Nigeria.
Folasade T. Ogunsola
Department of Medical Microbiology and Parasitology, College of Medicine of the University of Lagos, Nigeria.
Akitoye O. Coker
Department of Medical Microbiology and Parasitology, College of Medicine of the University of Lagos, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: Beta-lactamase production and subsequent resistance to β-lactam drugs has been a global concern in the treatment of Gram negative anaerobes. The aim of this study was to identify F. nucleatum strains producing Class D β-lactamase through the detection of FUS-1 (OXA-85) resistance gene.
Place and Duration of Study: Department of Preventive Dentistry, Lagos University Teaching Hospital, Idi-Araba, between February 2010 and November 2010.
Methodology: Twenty two oral clinical samples were obtained from patients with chronic periodontitis who admitted to previous use of amoxicillin. Antibacterial susceptibility of the bacterial isolates was determined by E-test on Brucella Blood agar. Amplification of the bacterial DNA was carried out by PCR using F. nucleatum species-specific primer, FUS-1 specific for blaFUS-1 and strain-specific primers for subspecies nucleatum,, fusiforme, polymorphum and vincentii.
Results: From the 19 samples collected, F. nucleatum was isolated, and the identity of the isolates was confirmed by PCR. Four of the isolates produced similar bands with the control strain, 3 (15.7%) strains were able to produce amplication with FUS-1 primer specific for blaFUS-1 gene found in β-lactamase producing F. nucleatum subsp. polymorphum.
Conclusion: This study shows the presence of class D β-lactamase producing F. nucleatum species in Nigeria.
Keywords: Beta-lactamase, resistance genes, Fusobacterium nucleatum, subspecies polymorphum