Molecular Characterisation of Extended-Spectrum Beta-lactamase Producing Escherichia coli Isolated from Cattle Faeces in Abidjan District, Ivory Coast
René Kouamé Yao *
Laboratory of Biochemical Pharmacodynamic, University Félix Houphouet-Boigny (UFHB), Ivory Coast.
Julien Kalpy Coulibaly
Department of Bacteriology and Virology, Pasteur Institute (IPCI), Ivory Coast.
Bertin Konan Tiekoura
Department of Bacteriology and Virology, Pasteur Institute (IPCI), Ivory Coast.
Félix Houphouët Yapi
Laboratory of Biochemical Pharmacodynamic, University Félix Houphouet-Boigny (UFHB), Ivory Coast.
Joseph Allico Djaman
Laboratory of Biochemical Pharmacodynamic, University Félix Houphouet-Boigny (UFHB), Ivory Coast.
*Author to whom correspondence should be addressed.
Abstract
Aims: To determine the frequency of Extended-Spectrum Beta-lactamase producing E. coli strains in cattle farms in Abidjan district (Ivory Coast).
Methodology: A total of 420 bovine faecal samples were collected in five townships in Abidjan district over six (6) month period from April to September 2016. ESBL-producing E. coli strains were isolated on Rapid E. coli 2 medium supplemented with 2 mg / L ceftazidime (antibiotic). The antibiotic resistance profile was evaluated by the diffusion method in agar media and the detection of strains of Extended-Spectrum Beta-lactamase producing E. coli was performed by the double-disk synergy test. Molecular detection of CTX-M, SHV and TEM genes was performed by polymerase chain reaction (PCR).
Results: Out of the 85 strains of E. coli isolated (20,2%), 45 strains were confirmed producing ESBL, a prevalence of 52,9%. The blaCTX-M, blaSHV and blaTEM genes were detected in 15 strains of Extended-Spectrum Beta-lactamase producing E. coli resistant to quinolone with respective frequencies of 73,3%, 66,7% and 100%.
Conclusion: This study showed a high prevalence of Extended-Spectrum Beta-lactamase producing E. coli strains in cattle faeces in the Abidjan district. A good use of antibiotics would be an alternative to overcome the problem of the emergence of Extended-Spectrum Beta-lactamase producing E. coli strains in farms
Keywords: E. coli, extended-spectrum beta-lactamase, cattle, faeces