Phenotypical Characterization of Pseudomonas aeruginosa Clinical Strains Isolated in Abidjan (Côte d'Ivoire)
Nicole Prisca Nieko Dangui Makaya *
Laboratoire de Génétique, Université Felix Houphouët Boigny, UFR Biosciences 22BP 582, Abidjan 22, Côte-d’Ivoire and Institut Pasteur, 01BP 490 Abidjan 01, Côte-d’Ivoire and Département de Sciences de la Vie, Université Marien Ngouabi, Ecole Normale Supérieure, Brazzaville, République du Congo and Laboratoire National de Santé Publique, B.P: 120, Brazzaville, République du Congo.
Nathalie Kouadio Guessennd
Institut Pasteur, 01BP 490 Abidjan 01, Côte-d’Ivoire.
Karine Manzan Kossia Gba
Laboratoire de Génétique, Université Felix Houphouët Boigny, UFR Biosciences 22BP 582, Abidjan 22, Côte-d’Ivoire and Institut Pasteur, 01BP 490 Abidjan 01, Côte-d’Ivoire.
Valérie Gbonon
Institut Pasteur, 01BP 490 Abidjan 01, Côte-d’Ivoire.
Bertin Konan Tiekoura
Institut Pasteur, 01BP 490 Abidjan 01, Côte-d’Ivoire.
Abalé Anatole Toty
Institut Pasteur, 01BP 490 Abidjan 01, Côte-d’Ivoire.
Joel Eric Tahou
Laboratoire de Génétique, Université Felix Houphouët Boigny, UFR Biosciences 22BP 582, Abidjan 22, Côte-d’Ivoire and Institut Pasteur, 01BP 490 Abidjan 01, Côte-d’Ivoire.
Simon-Pierre Assavon Nguetta
Laboratoire de Génétique, Université Felix Houphouët Boigny, UFR Biosciences 22BP 582, Abidjan 22, Côte-d’Ivoire.
Mireille Dosso
Institut Pasteur, 01BP 490 Abidjan 01, Côte-d’Ivoire.
*Author to whom correspondence should be addressed.
Abstract
Aims: Objective of this study was to determine the prevalence of clinical Pseudomonas aeruginosa strains expressing antibiotic resistance mechanisms of the family of β-lactams, aminoglycosides and fluoroquinolones in Abidjan.
Study Design: It is a retrospective study.
Place of Study: Laboratories of Bacteriology and virology of Pasteur Institut, Abidjan, Côte d'Ivoire.
Methodology: From January 2012 to December 2015 of P. aeruginosa strains from various hospital departments and pathological products of patients have been identified using conventional methods of Bacteriology and Maldi-Tof. Susceptibility strain to antibiotics was tested using the standard Mueller-Hinton agar diffusion susceptibility test method according to CA-SFM 2015.
Results: Two hundred and eight Pseudomonas aeruginosa strains were isolated which 35.83% coming from service of medicine. Among these strains 46.63% were isolated from abscess and 9.62% from urine. Antibiotics resistance profile showed a resistance of preferably β-lactams especially to ticarcillin (30.77%). Colistin was the most active antibiotic. Different types of phenotypes have been identified. Regarding β-lactams, the most observed phenotype were the wild ones with 61.06% of all the strains and 12.02% for the efflux phenotype. For aminoglycosides 69.23% were wild-type and 75.96% were wild type for fluoroquinolones.
Keywords: Mechanisms of resistance to antibiotics, phenotype